eNeurobiología - Revista electrónica

 

 

Mai M. Anwar¹*, Ola S. M. Ali², Laila Ahmed R.³, Badawi A. M.¹, Nadia A. Eltablawy¹

¹Department of Biochemistry, National Organization for Drug Control and Research (NODCAR), Egypt. ²Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, Egypt. ³Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Egypt

 

Abstract

Background: Alzheimer’s disease (AD) is characterized by severe accumulation of amyloid plaques and neurofibrillary tangles accompanied with cognitive dysfunction leading to major changes affecting the quality of patient daily life pattern. Objective: To investigate the effect of MSCs and/or milk kefir grains in LPS –induced AD in female albino rats in alternating manner. Materials and Methods: Sixty female albino rats were divided into equal six groups (ten rats each): group 1: healthy control; group 2: LPS-induced AD; group 3: LPS-induced AD rats received single intravenous injection of MSCs; group 4: LPS-induced AD rats received oral milk kefir; group 5: LPS-induced AD rats received a single intravenous injection of MSCs with a daily milk kefir grain administration for a month; group 6: Rats received kefir for one week prior to the induction of AD followed by a single intravenous injection of MSCs with a daily milk kefir grain administration for a month. AD was assessed by T maze behavioural test month after induction. Brain tissue was collected for monitoring BDNF, Bax, Bcl-2 and seladin-1 gene expression with the measurement of TNF-α, IL-10 and tissue cholesterol. Plasma lipid profile, GSH and MDA were also determined. Results: Revealed that significant elevation of lipid profile and oxidative stress in association with LPS-induction. BDNF, Bcl-2 and seladin-1 gene expression were significantly reduced in AD while Bax mRNA gene was significantly increased. Administration of kefir and /or MSCs suppressed LPS –induced AD. Conclusion: The pre and co-administration of kefir with MSCs could act as a potent modulator attenuating the underling pathological inflammatory process accompanying AD which results in the progression of brain damage.

Keywords: Alzheimer's disease, MSCs, kefir grains, LPS, Bax, BDNF, Seladin-1.

 

*Corresponding Author at: Department of Biochemistry, National Organization for Drug Control and Research (NODCAR), Egypt. Abo Hazem St., Pyramids Ave., Giza, Egypt. Phone: (+00201000993347) E-mail: mainwr@hotmail.com

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